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Follow all the action as it happens from the MGM Grand in Las Vegas as WBO Welterweight champion and Pound for Pound king, Manny Pacquiao takes on future Hall of Famer “Sugar” Shane Mosley. The action begins at 9pm est/6pm pac with a three fight undercard that will feature a 122 lb world championship between Wilfredo Vazquez Jr. and former three division champ Jorge Arce. Also the return of former Middleweight king Kelly Pavlik taking on undefeated Alfonso Lopez. The night will begin with Jr. Welterweight clash between Mike Alvarado and Ray Nahr.

12 ROUNDS–WBO WELTERWEIGHT CHAMPIONSHIP–MANNY PACQUIAO (52-3-2, 38 KO’S) VS SHANE MOSLEY (46-6-1, 39 KO’S)

12 ROUNDS–WBO SUPER BANTAMWEIGHT CHAMPIONSHIP–WILFREDO VAZQUEZ JR. (20-0-1, 17 KO’S) VS JORGE ARCE (56-6-2, 43 KO’S)

Round 1 Pacquiao lands a left…Pacquiao lands a left to the body…Mosley lands a right to the body..Pacquiao a left to the body…Pacquiao 10-9

Round 2 Mosley lands a 1-2; Pacquiao lands a left…Pacquiao lands a left…right hook…left…manny starting to get a rhythm…20-18 Pac

Round 3: Mosley gets in a right…STRAIGHT LEFT AND DOWN GOES MOSLEY...30-26 Pac

Round 4: Mosley lands a body shot and right…Pacquiao a right hook..Body shot…Mosley lands a combination..39-36 Pac

Round 5:Pacquiao lands a right hook…Pacquiao lands a body shot…head clash…Pacquiao lands a straight left…Mosley lands 2 rights to the body…Left hook…..49-46 Pac

Round 6 Pacquiao lands straight left to the body…Uppercut…combination…Mosley lands a right..59-55 Pac

Round 7: Pacquiao coming forward and Mosley doing nothing…69-64 Pac

Round 8 Mosley lands a right…Pacquiao lands a left to the head…Nice right by Mosley…78-74 Pac

Round 9 Pacquiao landing combinations…striaght left …right hooks…right hook..Mosley lands a right..88-83 Pac

Round 10 Combinations by knockdown…The feet get tangled BUT PACQUIAO IS DOWN AND RULED A KNOCKDOWN…Pacquiao drills Mosley with a right and left..Mosley holding on…97-93 Pac

Round 11 Pacquiao landing hard combinations throughout the round.,..Has Mosley in trouble a couple times. Mosley lands once nice counter right for Which lights yet another fire under Pacquiao who comes in with combinations…107-102 Pac

Round 12: Pacquiao lands a straight left..Straight left to the chin..2 right hooks..117-111 Pacquiao

119-108; 120-108; 120-107 FOR MANNY PACQUIAO

Round 1: Arce lands a good left hook…Good left to the body..Vazquez lands a looping right..Arce lands a left that has Vazquez holding on…big right…Vazquez lands a right…Arce lands to the body... Arce 10-9

Round 2:Nice combo from Vazquez…Arce lands a jab…left…right..20-18 Arce

Round 3: Blood from bridge of Arce’s nose..Double left from Arce…29-28 Arce

Round 4: Vazquez lands a counter left…Arce lands a right……BIG LEFT HOOK AND DOWN GOES ARCE….38-37 Vazquez

Round 5 Vazquez lands a right…Arce lands a left..Good body…trading body shots…Vazquez lands a left hook…another one..Arce lands a 3 punch combination..47-47

Round 6: Vazquez lands a right..Arce body..straight right…Vazquez lands 2 left hooks…57-56 Vazquez

Round 7: Going toe to toe on the ropes…Vazquez lands a right…67-65 Vazquez

Round 8: Aece just throwin and throwin…lands a left at the bell…76-75 Vazquez

Round 9: Arce lands a body...85-85

Round 10: Vazquez lands a good right…Hook and uppercut..Arce flurries…95-94 Vazquez

Round 11: Arce lands a left hook..They trade hooks…Arce lands a jumping left backs up Vazquez…Arce all over Vazquez…..104-104 Vazquez wobbles back to his corner

Round 12: Arce all over Vazquez…just unloading..VazQUEZ IN SERIOUS TROUBLE…THEY THROW IN THE TOWEL AND THE FIGHT IS OVER

10 ROUNDS–SUPER MIDDLEWEIGHTS–KELLY PAVLIK (36-2, 32 KO’S) VS ALFONSO LOPEZ (21-0, 16 KO’S)

ROUND 1: Pavlik lands a right…Good left hook…10-9 Pavlik

Round 2: Pavlik lands a left hook..Lopez comes back with a combination…19-19

Round 3: Pavlik works the body…Good right hand…Jab…29-28 Pavlik

Round 4: Pavlik lands a good combination…39-37 Pavlik

Round 5 Good combination from Lopez…Pavlik lands a short hook…48-47 Pavlik

Round 6: Good right from Pavlik..body shots has Lopez holding on..Big right…right to the body..58-56 Pavlik

Round 7: Pavlik gets in a left hook..Lopez working the combinations…Right from Pavlik…Pavlik warned for a low blow…Lopez lands a flurry..67-66 Pavlik

Round 8: Lopez lands a nice combination as Pavlik lands a left hook at the bell…76-76

Round 9: Pavlik lands a good right…Lopez lands a solid right and a short left hook…Left hook..86-85 Lopez

Round 10: Lopez lands a combination…Good body work…Big left by Pavlik buckles Lopez…another left hook..yet another…straight right…Lopping right..hard jab…Blood from right eye of Lopez…Right from Pavlik..95-95

95-95; 98-92; 99-91 PAVLIK BY MAJ DEC

12 Rounds–Jr. Welterweights–Mike Alvarado (29-0, 21 KO’s) vs Ray Narh (25-1, 21 KO’s)

Round 1: Nahr lands an uppercut…Jabbing…Alvarado lands an uppercut..10-0 Nahr

Round 2: Alvarado lands big jabs that shake Narh..19-19

Round 3: Alvarado jabbing…Right hand..another right…29-28 Alvarado

Round 4: NARH QUITS ON THE STOOL AND THE FIGHT IS STOPPED ALVARADO WINS BY TKO

Effect of maternal vitamin and mineral restrictions on the body fat content and adipocytokine levels of WNIN rat offspring.(Brief communication)

Nutrition & Metabolism October 15, 2007 | Lagishetty, Venu; Nandiwada, Vijaya Bhanu; Kalashikam, Rajender Rao; Manchala, Raghunath Authors: Venu Lagishetty (corresponding author) [1,2]; Vijaya Bhanu Nandiwada [1]; Rajender Rao Kalashikam [1]; Raghunath Manchala [1] Background Maternal under-nutrition impairs intrauterine development and increases adiposity, insulin resistance (IR) and associated metabolic disturbances in the later life of the offspring [1]. We reported earlier that chronic 50 percent restriction of minerals (MR) or vitamins (VR) in Wistar NIN (WNIN) rat dams increased the body fat percentage in offspring and decreased their insulin response to glucose challenge [2, 3]. We also reported that increased oxidative stress and impaired antioxidant status were associated with maternal VR but not MR induced changes in offspring [2, 3]. We now report whether or not increased body adiposity in the VR and MR offspring is associated with increased abdominal adiposity, altered expression of adipocytokines and insulin resistance.

Adipocytokines, the adipocyte derived bioactive molecules, mediate the systemic effects of obesity on health and regulate lipid metabolism as well as IR [4]. In fact the adipocytokine: leptin is important in the pathogenesis of eating disorders and obesity and mediates the neuro-endocrine response to food deprivation [5]. Overproduction of TNF-[alpha] modulates IR in obesity [6]. Furthermore, reduced expression of adiponectin and low plasma adiponectin levels are implicated in the pathogenesis of obesity and type 2 diabetes [7]. Indeed, mice lacking adiponectin display IR in some conditions [8, 9]. Considering that maternal VR and MR increased body adiposity and impaired glucose stimulated insulin secretion in the offspring, we have determined whether or not altered expression of adipocytokines was associated with these changes.

Methods Experimental Animals All animal experimental procedures were carried out in accordance with the ‘principles of laboratory animal care’ (NIH publication no. 85-23, revised 1985) and with the approval of the “Institute’s ethical committee on animal experiments” at National Institute of Nutrition, Hyderabad, India.

Female, weaning WNIN rats obtained from National Centre for Laboratory Animal Sciences, National Institute of Nutrition, Hyderabad, India were used in these experiments. The protocol used for animal grouping, feeding, breeding and maintenance was described by us previously [2, 3]. Briefly, the rats received for 12 weeks, a control (AIN 93G) diet or a similar diet with 50 percent (of control diet) restriction of mineral/vitamin mixture, mated with control males and continued on their respective diets through out gestation. At parturition, a third of the restricted dams were shifted to control diet while the remaining continued on restricted diets. Half the number of pups born to these restricted dams were weaned on to control diet while the other half continued on the respective restricted diet.

Blood was collected from the offspring (at six moths of age) after an overnight fast and used for the determination of plasma glucose, insulin and adipocytokines. Insulin resistance (HOMA IR) was computed and the body fat of the animals content was determined by the TOBEC method as described by us earlier [2, 3]. The animals were sacrificed by carbon dioxide inhalation, epididymal fad pads were excised quickly and their fresh weight determined.

Adipocytokine levels Plasma leptin and TNF – [alpha] concentrations were determined using a rat specific enzyme linked immunosorbent assay (R&D Systems, MN, USA). Rat specific RIA kit (Linco Research, MO, USA) was used to determine plasma adiponectin levels. The lower limits of detection were less than 22 pg/mL for leptin; 5 pg/mL for TNF-[alpha] and 1 ng/mL for adiponectin.

Statistical analysis Data was subjected to statistical analysis using SPSS package (version 10.0) and values presented as mean [+ -] SEM. Data was analyzed by one-way analysis of variance (ANOVA) followed by Post Hoc least significant difference (LSD) test. Wherever the heterogeneity was observed in the variance, differences between groups were tested by the non-parametric Mann – Whitney U test. The differences were considered significant only if p < 0.05.

Results Body fat content, epididymal fat pad weight and insulin resistance In line with the high body fat percentage observed in the offspring of VR and MR rat dams, the fresh weight of the epididymal fat pads was significantly higher in them (compared to controls) at six months of age (Table 1 &2). Rehabilitating VR mothers from parturition and their offspring from weaning (VSP) but not weaning VR offspring to control diet (VSW) reversed the body fat percentage and epididymal fat pad weight to levels comparable to controls (Table 1). While both the rehabilitation regimes mitigated the maternal MR induced increase in body fat percentage of the offspring only partially, MSP but not MSW could rescue the increased weight of the epididymal fat pad (Table 2).

Table 1 caption: Body weight, fat content and plasma adipocytokine levels in the offspring of vitamin restricted WNIN rat dams on postnatal day 180 [table “” not found /]
Table 2 caption: Body weight, fat content and plasma adipocytokine levels in the offspring of mineral restricted WNIN rat dams on postnatal day 180 [table “” not found /]
Despite their significant effects on the body fat percentage and epididymal fat pad weight in the offspring, neither maternal VR nor MR had any effect on their IR as assessed by HOMA IR (Table 1 &2). As a corollary, the two rehabilitation regimes had no effect on this parameter.

Effect of maternal vitamin restriction on adipocytokine levels Plasma adiponectin levels were significantly decreased (p < 0.05) and leptin levels increased (p < 0.05) in VR offspring (Table 1) compared to the control (VC) offspring on post natal day 180. However plasma TNF-[alpha] levels were comparable among different groups of the offspring. In line with the effects seen on the increased percentage of body fat and fresh weight of epididymal fat pads, VSP but not VSW corrected the changes in adiponectin and leptin levels by six months of age (Table 1). here body fat percentage calculator

Effect of maternal mineral restriction on adipocytokine levels Chronic MR in WNIN rat dams decreased both plasma adiponectin and leptin levels significantly (p < 0.05) in the offspring (Table 2) compared to controls (MC). Similar to the observations made in the offspring of rehabilitated VR dams, MSP but not MSW corrected these changes. Plasma TNF-[alpha] levels were comparable among the different groups of the offspring (Table 2).

Discussion Increased body adiposity and/or altered lipid metabolism not only precede but also lead to tissue insulin resistance [10, 11]. Inline with these reports, the offspring of both VR and MR rat dams had higher body fat percentage compared to controls. The increase in fresh weight of the epididymal fat pads suggests that the increased body fat content in the VR and MR offspring could be due to an increase in central adiposity, a hall mark feature associated with and predisposes individuals to IR later in life [11].

Similar to their effects on body fat percentage, rehabilitation of VR dams from parturition and their offspring from weaning but not weaning VR offspring to control diet rescued the increased epididymal fat pad weight. This observation stresses the importance of vitamin nutrition during lactation in programming the body composition of the offspring and is in line with our similar findings earlier [2]. That both the rehabilitation regimes mitigated the maternal MR induced increase in body fat percentage of the offspring only partially suggests its irreversibility to a great extent as compared to that induced by maternal VR. This is corroborated by the observation that even the increased weight of epididymal fat pads was corrected only partly by MSP but not MSW.

The significant decrease in plasma adiponectin and increase in leptin levels seen in VR offspring are in agreement with earlier reports which showed that similar changes were associated with increased body fat and IR [5, 7]. Although increased leptin levels are usually associated with increased food intake, food intake was not increased in the VR offspring suggesting that they were probably leptin resistant. These results in the VR offspring suggest an association between the altered expression of adiponectin and leptin and their high body adiposity, albeit their causal relationship remains to be delineated.

The decreased plasma adiponectin levels seen in MR offspring are in agreement with similar reports earlier [7] and corroborate the increased percentage of body fat observed in them. However, our observation that hypoleptinemia was associated with high body fat percentage in MR offspring is at variance with many earlier studies demonstrating an association between high plasma leptin levels and high percentage of body fat [5]. Further studies are clearly needed to delineate the role if any of the hypoleptinemia in maternal MR induced increase in body fat percentage in the offspring. Interestingly, hypoleptinemia observed here is in line with leptin deficiency reported in the genetically obese rodent models [12, 13] and also with the hypoleptinemia reported in type 1 and 2 diabetic patients [14].

Increased TNF-[alpha] levels are associated with increased adiposity and IR [6]. However chronic MR or VR did not alter plasma TNF-[alpha] levels significantly. This finding appears to rule out a role for TNF-[alpha] in maternal VR and MR induced changes in the body fat of the offspring.

That rehabilitation of VR and MR dams from parturition but not weaning the VR and MR pups to control diet could mitigate the changes in adipocytokine levels stresses the importance of vitamin and mineral nutrition during lactation in modulating adipocytokine expression in addition to the body adiposity of the offspring. However the finding that despite comparable (to controls) leptin levels, MSP offspring had higher percentage of body fat and epididymal fat pad weight is perplexing and suggests that hypoleptinemia and leptin resistance may both be involved in maternal MR induced changes in adiposity of the offspring.

Not withstanding the effects seen in the body fat percentage, epididymal fat pad weight and plasma adipocytokine levels, neither maternal VR nor MR had any significant effect on the IR status in the offspring as assessed by the HOMA IR values. Lack of any effect on the IR status of the offspring could be due to the shorter duration of VR/MR and/or the lower magnitude of VR/MR employed in these studies. Considering our earlier reports [2, 3] that maternal VR/MR irreversibly decreased insulin secretion by the offspring to a glucose challenge, the increased body fat percentage observed here in the VR/MR offspring suggests that maternal VR/MR could lead to a hyperglycemic state in the offspring at a later age. That the rehabilitation regimes had similar effects on plasma adipocytokines, body fat percentage and glucose stimulated insulin suggests that adipocytokines play an important role in maternal VR/MR induced programming of glucose stimulated insulin secretion and hence glucose metabolism in the offspring in addition to their body adiposity. go to site body fat percentage calculator

Conclusion The present observations indicate that increased central adiposity underlies the increased percentage of body fat in the offspring of VR and MR rat dams. Altered expression of adiponectin and leptin is associated with maternal VR and MR induced changes in the body adiposity (composition) of the offspring but maternal VR/MR differentially modulate their expression. That rehabilitation of restricted mothers from parturition but not weaning the restricted offspring to control diet could correct the changes in adipocytokine levels, epididymal fat pad eight and body fat percent may suggest a causal relationship, which however needs to be established. The results also suggest the importance of vitamin and mineral nutrition during lactation in modulating the body adiposity of the offspring, specially the central adiposity, a fore runner for IR and associated diseases in their later life.

Abbreviations MC: control diet through out MR: mineral restriction through out MSP: rehabilitation of mineral restricted mothers from parturition and their pups from weaning MSW: mineral restricted offspring weaned on to control diet.

VC: control diet through out VR: vitamin restriction through out VSP: rehabilitation of vitamin restricted mothers from parturition and their pups from weaning VSW: vitamin restricted offspring weaned on to control diet.

WNIN: Wistar NIN Competing interests The author(s) declare that they have no competing interests.

Authors’ contributions VL participated in the conception and design of the study, data collection, tissue sampling, statistical analysis and drafting of the manuscript.

VBN participated in animal experimentation and tissue sampling.

RRK participated in animal experimentation and carried out the immunoassays.

RM conceived of the study, and participated in its design, coordination and drafting of the manuscript.

All authors read and approved the final manuscript.

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Author Affiliation:

[1] Division of Endocrinology and Metabolism, National Institute of Nutrition, Hyderabad – 500 007 India [2] Division of Neonatology & Developmental Biology and the Neonatal Research Center, Department of Pediatrics, David Geffen School of Medicine UCLA, Los Angeles, CA, 90095, USA Article history:

Received Date: 7/12/2007 Accepted Date: 10/15/2007 Published Date: 10/15/2007 Article notes:

?© 2007 Lagishetty et al; licensee BioMed Central Ltd.

Lagishetty, Venu; Nandiwada, Vijaya Bhanu; Kalashikam, Rajender Rao; Manchala, Raghunath

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